A novel Gaussia luciferase immunoprecipitation assay for the detection of Getah virus antibodies in pigs.
Authors: Li Chenxi, Zhang Linjie, Guo Jinyao, Tian Tian, Tang Chenyang, Wei ZuZhang, Li Yanhua
Journal: Veterinary journal (London, England : 1997)
Summary
# Editorial Summary: Getah Virus Antibody Detection in Pigs Getah virus represents an emerging threat to pig production across Asia, causing pyrexia, neurological signs and reproductive failure with no current vaccine or antiviral intervention available in China. Researchers developed a luciferase immunoprecipitation system (LIPS) assay utilising Gaussia luciferase-tagged viral E2 protein to detect GETV-specific antibodies in porcine serum, optimising conditions with 10⁷ luminance units of antigen and 1:100 serum dilution to maximise assay performance. Testing against 106 positive and 325 negative pig samples established a signal-to-noise cutoff of 13.71, achieving 100% sensitivity and 99.69% specificity with no cross-reactivity to antibodies against ASFV, PRRSV, JEV, PRV or PCV2. Seroprevalence surveillance in Hebei Province demonstrated the assay's practical utility for field-based GETV screening. For equine practitioners, this advancement is relevant given that horses are also susceptible to GETV infection; the methodology represents a scalable diagnostic tool applicable to rapid herd health surveillance and may inform similar detection systems for equine populations, particularly in regions where arboviral disease epidemiology remains poorly characterised.
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Practical Takeaways
- •Not applicable to equine practice; this research focuses on porcine disease detection and is relevant only to swine practitioners and animal health officials managing GETV in pig populations
Key Findings
- •A Gaussia luciferase immunoprecipitation system (LIPS) assay achieved 99.69% specificity and 100% sensitivity for GETV antibody detection in pig serum using 106 GETV-positive and 325 GETV-negative samples
- •Optimal assay conditions were determined to be 10^7 luminance units of GLuc-E2 input with 1:100 serum dilution and a cutoff S/N ratio of 13.71
- •The LIPS assay demonstrated no cross-reactivity with antibodies against five major porcine pathogens including ASFV, PRRSV, JEV, PRV, and PCV2
- •Seroprevalence surveillance in Hebei Province, China validated the GLuc-E2-based LIPS assay as a reliable diagnostic tool for GETV detection in laboratory and field settings