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behaviour
nutrition
riding science
2021
Cohort Study

Microbial Prevalence and Antimicrobial Sensitivity in Equine Endometritis in Field Conditions.

Authors: Díaz-Bertrana María Luisa, Deleuze Stefan, Pitti Rios Lidia, Yeste Marc, Morales Fariña Inmaculada, Rivera Del Alamo Maria Montserrat

Journal: Animals : an open access journal from MDPI

Summary

# Editorial Summary Endometrial infection represents a leading cause of reproductive failure in mares, yet treatment decisions are frequently made without microbiological guidance. Díaz-Bertrán and colleagues cultured endometrial swabs from 363 subfertile mares with cytological and/or clinical evidence of endometritis, performing concurrent antimicrobial sensitivity testing on all isolates. Positive cultures were obtained in 89% of cases, with Staphylococcus, Streptococcus, Escherichia and Pseudomonas dominating the microbial landscape; E. coli alone accounted for 17.3% of infections. Critically, β-lactam antimicrobials—traditionally relied upon in equine practice—showed disappointing efficacy, with penicillin resistance exceeding 62% in Gram-positive organisms and 86% in Gram-negative isolates; amikacin demonstrated superior activity at 57.3% sensitivity across all cultures. These findings underscore the clinical importance of obtaining endometrial samples with antimicrobial susceptibility testing before initiating therapy, rather than deploying empirical broad-spectrum regimens that risk both therapeutic failure and antibiotic resistance development.

Read the full abstract on PubMed

Practical Takeaways

  • Culture and sensitivity testing are essential before treating equine endometritis, as β-lactam antimicrobials show poor efficacy against prevalent organisms
  • Avoid empirical penicillin therapy; amikacin, cefoxitin, or gentamicin are more likely to be effective based on this prevalence data
  • Nearly 9 in 10 subfertile mares with endometrial cytology findings will have positive bacterial cultures, justifying microbiological investigation in repetitively infertile mares

Key Findings

  • Positive bacterial culture obtained in 89% of 363 mares with repetitive infertility and endometrial cytology/vaginal discharge
  • Most prevalent genera were Staphylococcus (25.1%), Streptococcus (18.2%), Escherichia (17.3%), and Pseudomonas (12.1%)
  • Amikacin demonstrated highest antimicrobial efficacy (57.3%), followed by cefoxitin (48.6%) and gentamicin (48.3%)
  • Gram-positive bacteria showed high resistance to cephaloridine (77.3%), apramycin (70.8%), and penicillin (62.3%); Gram-negative highly resistant to penicillin (85.8%)

Conditions Studied

endometritisrepetitive infertilitypositive endometrial cytology