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2024
Case Report

Changes in saliva analytes in equine gastric ulcer syndrome (EGUS) after treatment: a pilot study

Authors: Muñoz-Prieto Alberto, Llamas-Amor Eva, Cerón José Joaquín, Hansen Sanni

Journal: Research in Veterinary Science

Summary

# Editorial Summary: Salivary Biomarkers in Equine Gastric Ulcer Syndrome Equine gastric ulcer syndrome affects the majority of racehorses and performance animals, with current diagnosis relying on endoscopy—an invasive and costly procedure that limits routine screening. Muñoz-Prieto and colleagues tested whether non-invasive salivary analytes could serve as biomarkers of treatment response in 28 horses treated with omeprazole for six weeks, using automated chemistry analysis to measure enzymes, proteins, minerals, and metabolites before and after therapy. Horses demonstrating successful treatment (ulcer score reduction, n=15) showed statistically significant increases in bicarbonate and urea alongside decreases in adenosine deaminase and creatine kinase, whilst the unsuccessful treatment group (n=13) exhibited no meaningful changes in these parameters. The practical value lies in the simplicity and speed of automated salivary analysis, offering equine professionals a potential chairside tool to evaluate treatment efficacy without repeated gastroscopy. Whilst results are promising, larger cohort studies are needed to validate these analytes as reliable clinical indicators and establish standardised thresholds for practitioners; nonetheless, this pilot opens a practical avenue for monitoring EGUS management in working and competitive horses.

Read the full abstract on PubMed

Practical Takeaways

  • Saliva sampling offers a non-invasive alternative to gastroscopy for monitoring EGUS treatment response, with specific analytes (bicarbonate, urea, ADA, CK) potentially indicating treatment success within 6 weeks
  • Automated saliva analysis for these biomarkers could be incorporated into routine practice for objective assessment of omeprazole efficacy without requiring endoscopic re-examination
  • Lack of salivary analyte changes may help identify horses with treatment-resistant EGUS earlier, allowing faster intervention with alternative therapies or diagnostic investigation

Key Findings

  • Horses with successful EGUS treatment showed significant increases in salivary bicarbonate and urea after 6 weeks of omeprazole therapy
  • Successful treatment group demonstrated significant decreases in salivary adenosine deaminase (ADA) and creatine kinase (CK)
  • Horses with unsuccessful treatment showed no significant changes in any salivary analytes measured
  • Salivary biomarkers can be measured rapidly using automated chemistry analyzers as potential indicators of EGUS treatment response

Conditions Studied

equine gastric ulcer syndrome (egus)equine squamous gastric disease (esgd)equine glandular gastric disease (eggd)

Related References

Towards the Identification of New Biomarkers in Saliva and Serum for Treatment Monitoring of Equine Gastric Ulcer Syndrome: A Liquid Proteomic Approach.

Muñoz-Prieto Alberto, Rubić Ivana, Rešetar Maslov Dina, González-Sánchez Juan Carlos, Mrljak Vladimir, Cerón Jose Joaquín, Hansen Sanni(2024)Animals : an open access journal from MDPI

Changes in Calprotectin (S100A8-A9) and Aldolase in the Saliva of Horses with Equine Gastric Ulcer Syndrome.

Muñoz-Prieto Alberto, Contreras-Aguilar María Dolores, Cerón José Joaquín, Ayala de la Peña Ignacio, Martín-Cuervo María, Eckersall Peter David, Holm Henriksen Ida-Marie, Tecles Fernando, Hansen Sanni(2023)Animals : an open access journal from MDPI

Evaluation of a Comprehensive Profile of Salivary Analytes for the Diagnosis of the Equine Gastric Ulcer Syndrome.

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Changes in Immunoglobulins G and A in the Saliva and Serum of Horses with Equine Gastric Ulcer Syndrome (EGUS) and Their Relationship with Other Immune and Redox Status Biomarkers

M. Botía, María Martín-Cuervo, S. Martínez-Subiela, J. Cerón, Ignacio Ayala, S. Hansen, A. Muñoz-Prieto(2024)Biology

Oxytocin in horse saliva: validation of a highly sensitive assay and a pilot report about changes in equine gastric ulcer syndrome.

Botía María, Muñoz-Prieto Alberto, Martínez-Subiela Silvia, Martín-Cuervo María, Hansen Sanni, Manteca Xavier, Cerón José Joaquín, López-Arjona Marina(2025)BMC veterinary research